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產品描敘
for RNA experiment, RNase deactivator. Synonym: Diethyl pyrocarbonate, DEP, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
產品說明
O(COOC2H5)2
M.W.: 162.14
Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.
Application
Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
For the treatment of water with DEPC, add 1 ml of DEPC to 1 liter bi-distilled water (0.1 % DEPC v/v) and stir over night. Autoclaving 20 minutes inactivates DEPC. DEPC reacts with water and hydrolyses to ethanol and CO2.
規格包裝
1.50ML
2. 50ML*2
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