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產品描敘
For RNA experiment or protein purification. Synonym: Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride, Guanidium chloride
產品說明
CH5N3·HCl
M.W.: 95.53
99.5% Min.
Loss on Drying: <0.5% Ash: <0.1% Ammonium Chloride: <0.5% Heavy Metals (as Pb): <0.001% Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase. Guanidine Hydrochloride denatures proteins and inhibits nucleases. Beta-Mercaptoethanol or DTT increase the denaturing activity of guanidine hydrochloride. Strongly hydrophobic proteins, like the membrane protein lipophilin, will not be denatured by guanidine hydrochloride. 包裝
- 1KG
- 1KG*5
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