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產品描敘
For DNA/RNA purification. Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase
產品說明
Molecular mass.: 14,300Da
Loss on Drying: <5% Residue on ignition: <0.10% Activity (25℃): >20,000U/mg
Optimal pH: 5.3-6.4
Optimal Temp.: 35℃
Inhibitors: imidazole, indole, N-acetyl glucosamine
Unit definition: The amount of enzyme causing a decrease in absorbance at 450nm of 0.001 per minute at 25℃ and pH 6.2 with Micrococcous Lysodeikticus as substrate.
Lysozyme hydrolyses the beta-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganism. The enzyme is present in many organism. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA. Another application is the lysis of bacteria for the preparation of bacterial RNA.
Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) 2001. Currrent Protocols in Molecular Biology. John Wiley & Sons, New York.
Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition. pp. A1.8+4.51. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
包裝:10G
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