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產品描敘
for DNA/RNA Purification. Synonym: Endopeptidase K
產品說明
Physical State: Solid (lyophilized powder)
Appearance : White Powder
Molecular Mass: 28,930 Da (amino acid sequence); 28,500 Da (SDS-PAGE)
Activity: >30 units/mg
Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification. Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis, inactivation of Rnase and Dnases, isolation of high molecular weight DNA, isolation of RNA or the isolation of plasmid and genomic DNA. Proteinase K is used for the isolation of native high molecular genomic nucleic acids.
Suggetion concentration:
Stock conc.:10-20mg/mL
Working conc.: 10-100ug/mL
Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York
Sambrook, J., Fritsch, E.F. & Maniatis, T.Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
包裝: 100mg or 500mg
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